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MCAT Question of the Day
A mammalian cytosolic enzyme is normally encoded by a gene containing several introns. Its catalytic activity does not require glycosylation or other eukaryote-specific posttranslational modifications. A student inserts the unmodified mammalian genomic sequence downstream of a strong bacterial promoter and ribosome-binding site in an E. coli expression plasmid.
Without using cDNA or otherwise engineering the coding region, what problem is most likely to prevent production of the normal amino acid sequence of the active mammalian enzyme?
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